Isolation and Characterization of Two cDNA Clones Encoding for Glutamate Dehydrogenase in Nicotiana plumbaginifolia
نویسندگان
چکیده
منابع مشابه
Isolation and Characterization of Expression cDNA Clones Encoding Antigens
The isolation of recombinant cDNA clones expressing antigens found in Onchocerca volvulus infective larvae is described. To isolate such clones, an expression cDNA library constructed from adult 0. volvulus RNA was screened with antiserum raised against infective larvae. One clone, designated XRAL-1 was characterized further. The recombinant antigen produced by XRAL-1 stimulates proliferation o...
متن کاملIsolation and characterization of cDNA clones encoding rat skeletal muscle peptidylarginine deiminase.
Various mammalian tissues contain protein-arginine deiminases (EC 3.5.3.15), which convert the arginine residues in normal peptide bonds to the citrulline residues in calcium ion-dependent manners. Here, we describe the complete primary structure of rat skeletal muscle peptidylarginine deiminase deduced from the sequences of its cDNA clones isolated by recombinant DNA technology. We have isolat...
متن کاملIsolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays.
We describe the isolation of cDNA clones encoding a p34cdc2 homologue from a higher plant, Zea mays (maize). A full-length cDNA clone, cdc2ZmA, was isolated, sequenced, and shown to complement a cdc28 mutation in Saccharomyces cerevisiae. Comparison of the deduced amino acid sequence of the maize p34cdc2 protein with other homologues showed that it was 64% identical to human p34cdc2 and 63% ide...
متن کاملIsolation and characterization of Borna disease agent cDNA clones.
Borna disease (BD) is a neurologic syndrome characterized by behavioral disturbances and the accumulation of specific proteins in limbic system neurons. A viral etiology has been proposed because BD can be induced in birds, rodents, and primates by inoculation with filtered brain homogenates from animals with BD. We report here the isolation and preliminary characterization of cDNA clones from ...
متن کاملCloning and characterization of a rice cDNA encoding glutamate decarboxylase.
In this study, we have isolated a rice (Oryza sativa L.) glutamate decarboxylase (RicGAD) clone from a root cDNA library, using a partial Arabidopsis thaliana GAD gene as a probe. The rice root cDNA library was constructed with mRNA, which had been derived from the roots of rice seedlings subjected to phosphorus deprivation. Nucleotide sequence analysis indicated that the RicGAD clone was 1,712...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 1999
ISSN: 0032-0781,1471-9053
DOI: 10.1093/oxfordjournals.pcp.a029547